In this work ZnO Nanoparticles were fabricated using Liquid Phase Pulse Laser Ablation technique and gives very simple, cheap and a single step method for the preparation of zinc oxide Nanoparticles with no need for any complex or expensive further steps. Also, It shows a long period of stability, less aggregation, non toxic and contamination colloidal Nanoparticles, In addition this work provides a controlled method to prepare Nanoparticles with a specific properties depended on the preparation conditions and laser parameters. In the present work, at which ablation of pure Zn metal target in DIW was accomplished using 9 nsec Q-switched Nd:YAG laser at (1.06 µm) laser wavelength , at different laser fluence and number of laser pulses, and the effect of these parameters on optical, photoluminescence, amount of ablated material, structural and surface morphology have been studied. The atomic absorption result shows that the amount of the ablated material is directly proportional to the laser fluence and number of laser pulses. The UV-Visible show a red shift in the absorption spectra related to the shift in the energy gap due to the size increase in the particle size with to higher laser fluence. A blue shift was however recognized with a larger number of laser pulses. The photoluminescence result gives a blue shift with the decrease in laser fluence and number of laser pulses. The X-Ray diffraction pattern revealed the presence of (100) plane related to ZnO wurtize structure. The Fourier transform infrared spectroscopy result shows that the intensity of the Zn-O bond vibrational mode is proportional directly to the laser fluence and the number of laser pulses. Grain size of the obtained NPs are found to increase with laser fluence and decreased with the number of laser pulses as shown by the AFM result.